Analysis by Liquid Chromatography-Mass Spectrometry
Liquid chromatography–mass spectrometry (LC-MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). Coupled chromatography - MS systems are popular in chemical analysis because the individual capabilities of each technique are enhanced synergistically. While liquid chromatography separates mixtures with multiple components, mass spectrometry provides structural identity of the individual components with high molecular specificity and detection sensitivity. This tandem technique can be used to analyze biochemical, organic, and inorganic compounds commonly found in complex samples of environmental and biological origin. Therefore, LC-MS may be applied in a wide range of sectors including biotechnology, environment monitoring, food processing, pharmaceutical, agrochemical, and cosmetic industries.
In addition to the liquid chromatography and mass spectrometry devices, an LC-MS system contains an interface that efficiently transfers the separated components from the LC column into the MS ion source. Overall, the interface is a mechanically simple part of the LC-MS system that transfers the maximum amount of analyte, removes a significant portion of the mobile phase used in LC, and preserves the chemical identity of the chromatography products (chemically inert). Most extensively applied LC-MS interfaces are currently based on atmospheric pressure ionization (API) strategies like electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photo-ionization (APPI).
Impact Analytical has multiple LC-MS/MS and exact mass Q-TOF LC-MS systems for the analysis small molecules and of semi- and nonvolatile materials in various matrices. For bioanalysis, we offer state of the art instrumentation and very low quantitation limits (femtogram per mL). Our in-house databases have hundreds of compounds including polymer additives, polymer extractables, pharmaceutical impurities, surfactants, degradation products, and color bodies. We offer electrospray (ESI) and atmospheric chemical ionization (APCI) modes, in both positive and negative. All equipment is qualified for testing under cGMP and GLP (FDA, EPA) guidelines, if needed.